Device

Part:BBa_K554012

Designed by: UNICAMP EMSE Brazil team   Group: iGEM11_UNICAMP-EMSE_Brazil   (2011-09-26)

SoxS GFP HlyA device

SoxS GFP HlyA device is part of the Device 2 testing: SoxR/SoxS system regulating GFP production. It is composed by SoxS Promoter linked to RBS, GFP gene, HlyA and Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 712


Usage and Biology

In order to test the ability of Jedi Bacteria in sensing NO levels and activating genes linked to SoxS promoter, we built a Device testing (Click here to see the experimental results), with GFP linked to SoxS promoter, as it is shown in the following schema (Figure 1):

UNICAMP EMSE GFP SOX device.jpg
Figure 1: Testing [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_2:_NO_sensor.2FIL-10_producer Device 2] through replacement of IL-10 to GFP.
[edit]
Categories
//collections/immune_regulation/devices
Parameters
None